TEV & SUMO Proteases: tag removal made simple!

TEV Protease – the gold standard for fusion tag cleavage

• Cleavage site: ENLYFQ↓X* – exceptional sequence specificity

• Benefits: User-friendly, minimal side activity

• Ideal for: Removal of C-terminal His-, GST-, or MBP-tags post-affinity purification

TEV Protease (recognition site E-N-L-Y-F-Q↓X*) is a very specific protease from Tobacco Etch Virus (TEV) that is used for cleavage of recombinant fusion proteins and removal of tags in vitro and in vivo.

* X = S, G, A, M, C or H

SUMO Protease – “scarless” and highly precise

• Specificity: Recognizes and cleaves the structured SUMO tag without leaving extra residues

• Benefits: No unwanted “scars” at your protein’s N-terminus, operates in gentle conditions

• Ideal for: Highly expressed proteins due to high specific activity

SUMO protease is a highly specific enzyme that recognizes the tertiary structure of the SUMO (Small Ubiquitin-like Modifier) tag and cleaves precisely at the C-terminus, usually after the conserved Gly-Gly motif (...-Gly–Gly↓X) at the native junction between SUMO and the target protein, leaving no additional amino acids. It is commonly derived from the yeast Saccharomyces cerevisiae and is widely used in recombinant protein purification for efficient and “scarless” tag removal.

Jena Bioscience offers TEV Protease in 2 concentrations as standard or highly concentrated solution and SUMO Protease at competitive price.

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