Bright & Persistent Red Fluorescent Reporter tdTomato Self-Amplifying RNA

dTomato Self-Amplifying RNA encodes a bright red fluorescent protein, emitting light at 581 nm. It is ideal for tracking gene expression, protein localization, and live cell imaging. As a self-amplifying RNA, it offers enhanced, prolonged expression with lower doses, making it efficient for extended studies. Croyez’s tdTomato saRNA was generated through in vitro transcription, and these mRNAs are then fortified at their 5' end by modified nucleotide capping, known as Cap1. To mimic the characteristics of fully processed mature mRNAs, we incorporate a poly(A) tail at the 3' end and optimize the mRNAs to enhance stability and overall performance. This ensures that the mRNAs function similarly to naturally occurring mature mRNAs in cells.