Featured Article - October 2025

JENA BIOSCIENCE NEWS

In a recent Cell paper[1], Glaser, Uhlmann and colleagues show that the replication machinery (replisome) can physically pass through the ring-shaped cohesin complex during DNA replication. We are honored that various nucleotide reagents from Jena Bioscience contributed to the success of this ambitious group of scientists, helping them to truly advance in the field of replication & chromatin research.

Using reconstituted biochemical systems and single-molecule fluorescence microscopy, they show that the replisome, aided by DNA polymerases α and ε, can move through cohesin without disassembling it. This ensures that both sister chromatids remain enclosed within the same ring for faithful chromosome segregation.

aithful chromosome segregation.

Why it matters:

It breaks with the assumption that cohesin must open or be removed for replication to proceed.

The study provides the first direct evidence that the replisome can pass through cohesin intact, redefining how replication and cohesin are connected.

By showing that polymerases α and ε enable this passage, it links DNA replication enzymes directly to chromosome architecture - bridging the processes of replication and segregation in a surprisingly simple way.

Read the full article: https://www.bioz.com/articles/showDocs/

Here is an overview of the products used in this study:

Product	Cat. No.	Amount / Reactions
ATPγS	NU-406-5	5 mg
NU-406-25	25 mg
NU-406-50	50 mg
AppNHp (AMPPNP)	NU-407-10	10 mg
NU-407-50	50 mg
dNTP Bundle	NU-1005S	4 x 200 µl (4 x 20 µmol)
NU-1005L	4 x 1 ml (4 x 100 µmol)
ATP - Solution	NU-1010	1 ml (100 mM)
NTP Bundle	NU-1014S	4 x 200 µl (4 x 20 µmol)
NU-1014L	4 x 1 ml (4 x 100 µmol)

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